Fluorescence Emission Correction (350-1100nm)
The response of the optical (optical fibers, grating, mirrors & lens) and electrical components (detector) that make up the detection system in a fluorescence spectrometer depends on the wavelength. As a result, the spectrometer detects different wavelengths of light with varying efficiency.
To eliminate the wavelength dependence of the detection system, an emission correction factor must be applied to the measured spectra. These correction factors are determined using calibration lamps with a known output. For a better understanding of its application, we recommend watching our Fluorescence Module Video.
The response of the optical (optical fibers, grating, mirrors & lens) and electrical components (detector) that make up the detection system in a fluorescence spectrometer depends on the wavelength. As a result, the spectrometer detects different wavelengths of light with varying efficiency.
To eliminate the wavelength dependence of the detection system, an emission correction factor must be applied to the measured spectra. These correction factors are determined using calibration lamps with a known output. For a better understanding of its application, we recommend watching our Fluorescence Module Video.
The response of the optical (optical fibers, grating, mirrors & lens) and electrical components (detector) that make up the detection system in a fluorescence spectrometer depends on the wavelength. As a result, the spectrometer detects different wavelengths of light with varying efficiency.
To eliminate the wavelength dependence of the detection system, an emission correction factor must be applied to the measured spectra. These correction factors are determined using calibration lamps with a known output. For a better understanding of its application, we recommend watching our Fluorescence Module Video.